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Image Search Results
Journal: Communications Biology
Article Title: Dexamethasone and lactoferrin induced PMN-MDSCs relieved inflammatory adverse events of anti-cancer therapy without tumor promotion
doi: 10.1038/s42003-021-01769-z
Figure Lengend Snippet: a – c BM cells from 6–8-week-old mice were treated with lactoferrin, DXM, combination of DXM and lactoferrin (D/L), or PBS vehicle (Con) in the presence of GM-CSF and IL-6 for 3 d. PMN-MDSCs were analyzed using a flow cytometer. a Typical example of flow cytometric analysis. b Absolute numbers and proportion of cells ( n = 6). c Antibacterial activity of Con PMN-MDSCs and D/L PMN-MDSCs isolated from PBS- or D/L-treated mice BM cells against E. coli ( n = 6 biologically independent samples). d – f Suppressive activity of Con PMN-MDSCs and D/L PMN-MDSCs against proliferation of T cells from WT mice stimulated with anti-CD3/anti-CD28 or OT-I–derived CD8 + T cells stimulated with cognate peptide were tested by CFSE. Non-stimulated T cells (No-sti), stimulated T cells without PMN-MDSCs (No-MDSC), and tumor PMN-MDSCs from spleen of B16 bearing mice (tumor) were used as controls. d Typical example of flow cytometric analysis. e Statistical analysis. f IFN-γ level (in media) analyzed by ELISA ( n = 6 biologically independent samples). Error bars displayed the mean and standard deviation.
Article Snippet:
Techniques: Flow Cytometry, Activity Assay, Isolation, Derivative Assay, Enzyme-linked Immunosorbent Assay, Standard Deviation
Journal: Communications Biology
Article Title: Dexamethasone and lactoferrin induced PMN-MDSCs relieved inflammatory adverse events of anti-cancer therapy without tumor promotion
doi: 10.1038/s42003-021-01769-z
Figure Lengend Snippet: a , b PBMCs from an adult healthy donor were treated with lactoferrin, DXM, combination of DXM and lactoferrin (D/L), or PBS vehicle (Con) in the presence of GM-CSF and IL-6 for 3 d. PMN-MDSCs were analyzed using a flow cytometer. a Typical example of flow cytometric analysis. b Absolute numbers and proportion ( n = 6 biologically independent samples). c Antibacterial activity of Con PMN-MDSCs and D/L PMN-MDSCs isolated from PBS- or D/L-treated PBMCs against E. coli ( n = 6 biologically independent samples). d – f Suppressive activity of Con PMN-MDSCs and D/L PMN-MDSCs against proliferation of T cells from PBMC stimulated with anti-CD3/anti-CD28 were tested by CFSE. Non-stimulated T cells (No-sti), stimulated T cells without PMN-MDSC (No-MDSC), and tumor PMN-MDSCs from hepatocellular carcinoma patients were used as controls. d Typical example of flow cytometric analysis. e Statistical analysis. f IFN-γ level (in media) analyzed by ELISA ( n = 6 biologically independent samples). Error bars displayed the mean and standard deviation.
Article Snippet:
Techniques: Flow Cytometry, Activity Assay, Isolation, Enzyme-linked Immunosorbent Assay, Standard Deviation
Journal: Communications Biology
Article Title: Dexamethasone and lactoferrin induced PMN-MDSCs relieved inflammatory adverse events of anti-cancer therapy without tumor promotion
doi: 10.1038/s42003-021-01769-z
Figure Lengend Snippet: a Diagram of the experimental design. Adult mice were intraperitoneally sensitized with 5 μg/g OVA emulsified in 200 μg/g of aluminum hydroxide on days 1 and 8 after tumor cell implantation, followed by intranasal challenge with OVA (5 μg/μl of PBS) daily on day 18 through 22. One day after the last challenge, the mice were sacrificed, and the lung inflammation was examined. For PMN-MDSC transfer experiments, 1 × 10 6 cells of Con PMN-MDSC, DXM/lactoferrin (D/L) PMN-MDSC, tumor PMN-MDSC, or vehicle control (PBS) were administered i.v. before the first challenge every other day three times. DXM was administered at 1 mg/kg/day followed by every challenge in the indicated group. b Total cell counts in the bronchoalveolar lavage fluid (BALF). c Eosinophil granulocyte percentage in BALF cells. d Representative images of hematoxylin and eosin-stained sections of the lungs and e Histopathological scores. f IL-4 level in BALF by ELISA. g Tumor growth. h Typical example of flow cytometric analysis and i statistical analysis of PMN-MDSCs in the lungs ( n = 6 biologically independent animals). Error bars displayed the mean and standard deviation.
Article Snippet:
Techniques: Control, Staining, Enzyme-linked Immunosorbent Assay, Standard Deviation